Application of (e,2e) Spectroscopy to the Electronic Structure of Valence Electrons in Crystalline and Amorphous Solids

This review presents theoretical and experimental aspects of (e,2e) spectroscopy specific to the study of crystalline and amorphous solids. The cross section for (e,2e) scattering is proportional to the spectral momentum density of the ejected electron under certain approximations. The theoretical framework for interpreting (e,2e) measurements is summarized here and general properties of the spectral momentum density of solids are discussed. Different designs of the (e,2e) spectrometer are described and sample preparation techniques are briefly reviewed. A summary of recent (e,2e) experiments on the electronic structure of valence electrons in graphite, ion sputtered and evaporated amorphous carbon, and aluminum-aluminum oxide films is given. The review concludes with a discussion of an ideal (e,2e) spectrometer for studying bulk properties of solids

HoxA9 binds and represses the Cebpa +8 kb enhancer

C/EBPα plays a key role in specifying myeloid lineage development. HoxA9 is expressed in myeloid progenitors, with its level diminishing during myeloid maturation, and HOXA9 is over-expressed in a majority of acute myeloid leukemia cases, including those expressing NUP98-HOXD13. The objective of this study was to determine whether HoxA9 directly represses Cebpa gene expression. We find 4-fold increased HoxA9 and 5-fold reduced Cebpa in marrow common myeloid and LSK progenitors from Vav-NUP98-HOXD13 transgenic mice. Conversely, HoxA9 decreases 5-fold while Cebpa increases during granulocytic differentiation of 32Dcl3 myeloid cells. Activation of exogenous HoxA9-ER in 32Dcl3 cells reduces Cebpa mRNA even in the presence of cycloheximide, suggesting direct repression. Cebpa transcription in murine myeloid cells is regulated by a hematopoietic-specific +37 kb enhancer and by a more widely active +8 kb enhancer. ChIP-Seq analysis of primary myeloid progenitor cells expressing exogenous HoxA9 or HoxA9-ER demonstrates that HoxA9 localizes to both the +8 kb and +37 kb Cebpa enhancers. Gel shift analysis demonstrates HoxA9 binding to three consensus sites in the +8 kb enhancer, but no affinity for the single near-consensus site present in the +37 kb enhancer. Activity of a Cebpa +8 kb enhancer/promoter-luciferase reporter in 32Dcl3 or MOLM14 myeloid cells is increased ~2-fold by mutation of its three HOXA9-binding sites, suggesting that endogenous HoxA9 represses +8 kb Cebpa enhancer activity. In contrast, mutation of five C/EBPα-binding sites in the +8 kb enhancer reduces activity 3-fold. Finally, expression of a +37 kb enhancer/promoter-hCD4 transgene reporter is reduced ~2-fold in marrow common myeloid progenitors when the Vav-NUP98-HOXD13 transgene is introduced. Overall, these data support the conclusion that HoxA9 represses Cebpa expression, at least in part via inhibition of its +8 kb enhancer, potentially allowing normal myeloid progenitors to maintain immaturity and contributing to the pathogenesis of acute myeloid leukemia associated with increased HOXA9

An Spectrometer for Investigating the Spectral Momentum Density of Thin Films

An (e, 2e) spectrometer has been constructed for detecting scattered and recoiling electrons from a thin solid film. The cross section for observing the two electrons in coincidence is proportional to the spectral momentum density of the target. In this spectrometer the energy of the incident electron beam is 25 keV and the beam current is approximately 40 μA. The energy resolution (FWHM) is ≤4 eV. The momentum resolution (FWHM) can be varied from 0.2 to 1.0 Å−1. Preliminary coincidence data from an amorphous carbon film are presented

Bacterial susceptibility and resistance to modelin-5.

Modelin-5 (M5-NH ) killed with a minimum lethal concentration (MLC) of 5.86 μM and strongly bound its cytoplasmic membrane (CM) with a of 23.5 μM. The peptide adopted high levels of amphiphilic α-helical structure (75.0%) and penetrated the CM hydrophobic core (8.0 mN m ). This insertion destabilised CM structure increased lipid packing and decreased fluidity (Δ 0) and promoted only low levels of lysis (24.3%). The insertion and lysis of the CM by M5-NH showed a strong negative correlation with its lysyl phosphatidylglycerol (Lys-PG) content ( > 0.98). In combination, these data suggested that Lys-PG mediated mechanisms inhibited the membranolytic action of M5-NH against , thereby rendering the organism resistant to the peptide. These results are discussed in relation to structure/function relationships of M5-NH and CM lipids that underpin bacterial susceptibility and resistance to the peptide

The effect of anthelmintic treatment during pregnancy on HIV plasma viral load: results from a randomized, double-blind, placebo-controlled trial in Uganda.

BACKGROUND: To investigate the effect of helminth infections and their treatment during pregnancy on HIV load, we conducted a 2 × 2 factorial randomized controlled trial of albendazole versus placebo and praziquantel versus placebo in pregnant women in Entebbe, Uganda. METHODS: Two hundred sixty-four HIV-infected pregnant women from the Entebbe Mother and Baby Study (ISRCTN 32849447) were included in this analysis. Women were tested for helminth infections at enrollment, and mean HIV load was compared between infected and uninfected groups. The effect of anthelmintic treatment on HIV load was evaluated at 6 weeks after treatment and at delivery using linear regression and adjusting for enrollment viral load. RESULTS: Hookworm and Trichuris infections were associated with higher mean viral load at enrollment [adjusted mean difference 0.24 log10 copies/mL, 95% confidence interval (CI): 0.01 to 0.47, P = 0.03, and 0.37 log(10) copies/mL, 95% CI: 0.00 to 0.74, P = 0.05, respectively]. There were no associations between viral load and other helminth species. There was some evidence that albendazole reduced viral load at 6 weeks after treatment (adjusted mean difference -0.17, 95% CI: -0.36 to 0.01, P = 0.07); however, this effect did not differ according to mother's hookworm infection status and had diminished at delivery (adjusted mean difference -0.11, 95% CI: -0.28 to 0.07, P = 0.23). There was no effect of praziquantel treatment on HIV load at any time point. CONCLUSIONS: Infection with some soil-transmitted helminth species is associated with increased HIV load in pregnancy. Treatment with albendazole causes a small decrease in HIV load; however, this may not represent a direct effect of worm removal

Maximally Rotating Supermassive Stars at the Onset of Collapse: Effects of Gas Pressure

The "direct collapse" scenario has emerged as a promising evolutionary track for the formation of supermassive black holes early in the Universe. In an idealized version of such a scenario, a uniformly rotating supermassive star spinning at the mass-shedding (Keplerian) limit collapses gravitationally after it reaches a critical configuration. Under the assumption that the gas is dominated by radiation pressure, this critical configuration is characterized by unique values of the dimensionless parameters $J/M^2$ and $R_p/M$, where $J$ is the angular momentum, $R_p$ the polar radius, and $M$ the mass. Motivated by a previous perturbative treatment we adopt a fully nonlinear approach to evaluate the effects of gas pressure on these dimensionless parameters for a large range of masses. We find that gas pressure has a significant effect on the critical configuration even for stellar masses as large as $M \simeq 10^6 M_{\odot}$. We also calibrate two approximate treatments of the gas pressure perturbation in a comparison with the exact treatment, and find that one commonly used approximation in particular results in increasing deviations from the exact treatment as the mass decreases, and the effects of gas pressure increase. The other approximation, however, proves to be quite robust for all masses $M \gtrsim 10^4 M_{\odot}$.Comment: 13 pages, 7 figures, version accepted for publication in MNRA

The Effect of Gender Stereotypes in Language on Attitudes Toward Speakers

This study uses a matched guise technique to elicit evaluations of men and women from participants based solely on what they hear. Four speakers (two men and two men) created two recordings, one in which they incorporated "women's language" into their speech and the other using "standard" language. One hundred university students listened to each recording and evaluated the speaker in terms of twelve personality traits. Results showed a significant difference in how male and female speakers were perceived, regardless of the language style they employed. Women's language and "standard" language were also perceived differently regardless of speaker gender

A visualisation tool to analyse usage of web-based interventions: The example of Positive Online Weight Reduction (POWeR)

Background: Attrition is a significant problem in web-based interventions. Consequently, research aims to identify the relation between web usage and benefit from such interventions. We have developed a visualisation tool that enables researchers to more easily examine large data sets on intervention usage that can be difficult to make sense of using traditional descriptive or statistical techniques alone. Objectives: This paper demonstrates how the visualisation tool was used to explore patterns in participants’ use of a web-based weight management intervention (POWeR: Positive Online Weight Reduction). We also demonstrate how the visualisation tool can be used to inform subsequent statistical analyses of the association between usage patterns, participant characteristics, and intervention outcome. Methods: The visualisation tool was used to analyse data from 132 participants who had accessed at least one session of the POWeR intervention. Results: There was a drop in usage of optional sessions after participants had accessed the initial, core POWeR sessions, but many users nevertheless continued to complete goal and weight review. POWeR tools relating to the food diary and steps diary were re-used most often. Differences in participant characteristics and usage of other intervention components were identified between participants who did and did not choose to access optional POWeR sessions (in addition to the initial core sessions) or re-use the food and steps diary. Re-use of the steps diary and the getting support tools was associated with greater weight loss. Conclusions: The visualisation tool provided a quick and efficient method for exploring patterns of web usage, which enabled further analyses of whether different usage patterns were associated with participant characteristics or differences in intervention outcome. Further usage of visualisation techniques is recommended in order to 1) make sense of large data sets more quickly and efficiently, 2) determine the likely active ingredients in web-based interventions, and thereby enhance the benefit they may provide and 3) inform (re-)design of future interventions to promote greater use and engagement by enabling users to easily access valued intervention content/tools

Flight Experiments on the Effects of Contamination on Electron Emission of Materials

We report on a study of the effects of prolonged exposure to the space environment and of chargeenhanced contamination on the electron emission and resistivity of spacecraft materials. The State of Utah Space Environment & Contamination Study (SUSpECS) was deployed on the International Space Station (ISS) in March 2008 onboard the MISSE-6 payload during STS-123. The Materials International Space Station Experiment (MISSE-6) program is designed to characterize the performance of candidate new space materials over the course of its ~17 month exposure to the LEO environment, with a target return date of August 2009 on STS-127. Approximately 165 samples are mounted on three separate SUSpECS panels in the ram and wake sides on the ISS. They have been carefully chosen to provide needed information for different ongoing studies and a broad cross-section of prototypical materials used on the exteriors of spacecrafts. Design of the sample panels are detailed, including a three tiered configuration intended to provide variable atomic oxygen and ultraviolet radiation exposure. The methods used to simulate charge enhanced contamination by actively biasing samples to low positive and negative voltages are also described. A primary emphasis of SUSpECS is the study of modifications to the electron emission resulting from exposure to the space plasma environment and to environmental contamination. There is presently little available data related to the effects of sample deterioration and contamination on emission properties for materials actually flown in space. Electron emission and transport properties of materials are key in determining the amount of charge build-up and the time for the charge to dissipate, as well as the likelihood of deleterious spacecraft charging effects. Such materials properties are essential parameters in modeling spacecraft charging with engineering tools like NASCAP-2K code. SUSpECS studies will test the validity of our predictions from ground-based studies that very thin layers of contamination can lead to severe charging effects under certain circumstance. Electron-, ion-, and photon-induced electron emission yield curves, crossover energies and emission spectra, as well as resistivity and dielectric strength, have been tested for most SUSpECS samples in their pristine conditions before flight. These measurements will be compared with post-flight measurements. Additional pre- and post-flight characterization measurements include optical and electron microscopy, reflection spectroscopy, emissivity and Auger electron spectroscopy

A Full-Sky H-alpha Template for Microwave Foreground Prediction

A full-sky H-alpha map with 6 arcmin (FWHM) resolution is presented. This map is a composite of the Virginia Tech Spectral line Survey (VTSS) in the north and the Southern H-Alpha Sky Survey Atlas (SHASSA) in the south. The Wisconsin H-Alpha Mapper (WHAM) survey provides a stable zero-point over 3/4 of the sky on a 1 degree scale. This composite map can be used to provide limits on thermal bremsstrahlung (free-free emission) from ionized gas known to contaminate microwave-background data. The map is available on the WWW for public use.Comment: 20 pages, 7 figures, ApJS in press, get data at http://skymaps.inf